Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs.

ABSTRACT:

Background

microRNAs (miRNA) are short, endogenous transcripts that negatively regulate the expression of specific mRNA targets. miRNAs are found both in tissues and body fluids such as plasma. A major perspective for the use of miRNAs in the clinical setting is as diagnostic plasma markers for neoplasia. While miRNAs are abundant in tissues, they are often scarce in plasma. For quantification of miRNA in plasma it is therefore of importance to use a platform with high sensitivity and linear performance in the low concentration range. This motivated us to evaluate the performance of three commonly used commercial miRNA quantification platforms: GeneChip miRNA 2.0 Array, miRCURY Ready-to-Use PCR, Human panel I+II V1.M, and TaqMan Human MicroRNA Array v3.0.

Results

Using synth

SUBMITTER: Jensen SG 

PROVIDER: S-EPMC3184117 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature