Macrophage endocytic trafficking of antiretroviral nanoparticles.

ABSTRACT:

Aim

Nanoformulated antiretroviral therapy can improve drug compliance for people infected with HIV. Additional benefits would include specific drug deliveries to viral reservoirs and reduction in systemic toxicities.

Methods

In this article, we describe mechanisms of crystalline antiretroviral nanoparticle (NP) uptake, intracellular trafficking and release in human monocyte-derived macrophages.

Results

Following clathrin-dependent endocytosis NPs bypassed lysosomal degradation by sorting from early endosomes to recycling endosome pathways. Disruption of this pathway by siRNAs or brefeldin-A impaired particle release. Proteomic and biological analysis demonstrated that particle recycling was primarily Rab11 regulated. Particles were released intact and retained complet

SUBMITTER: Kadiu I 

PROVIDER: S-EPMC3184214 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature