Dividing to unveil protein microheterogeneities: traveling wave ion mobility study.

ABSTRACT: Overexpression of a protein in a foreign host is often the only route toward an exhaustive characterization, especially when purification from the natural source(s) is hardly achievable. The key issue in these studies relies on quality control of the purified recombinant protein to precisely determining its identity as well as any undesirable microheterogeneities. While standard proteomics approaches preclude unbiased search for modifications, the optional technique of top-down tandem mass spectrometry (MSMS) requires the use of highly accurate and highly resolved experiments to reveal subtle sequence modifications. In the present study, the top-down MSMS approach combined with traveling wave ion mobility (TWIM) separation was evaluated for its ability to achieve high sequence coverage and

SUBMITTER: Halgand F 

PROVIDER: S-EPMC3184335 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature