Individual organelle pH determinations of magnetically enriched endocytic organelles via laser-induced fluorescence detection.

ABSTRACT: The analysis of biotransformations that occur in lysosomes and other endocytic organelles is critical to studies on intracellular degradation, nutrient recycling, and lysosomal storage disorders. Such analyses require bioactive organelle preparations that are devoid of other contaminating organelles. Commonly used differential centrifugation techniques produce impure fractions and may not be compatible with microscale separation platforms. Density gradient centrifugation procedures reduce the level of impurities but may compromise bioactivity. Here we report on simple magnetic setup and a procedure that produce highly enriched bioactive organelles based on their magnetic capture as they traveled through open tubes. Following capture, in-line laser-induced fluorecence detection (LIF) determ

SUBMITTER: Satori CP 

PROVIDER: S-EPMC3184341 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature