Comprehensive exon array data processing method for quantitative analysis of alternative spliced variants.

ABSTRACT: Alternative splicing of pre-mRNA generates protein diversity. Dysfunction of splicing machinery and expression of specific transcripts has been linked to cancer progression and drug response. Exon microarray technology enables genome-wide quantification of expression levels of the majority of exons and facilitates the discovery of alternative splicing events. Analysis of exon array data is more challenging than the analysis of gene expression data and there is a need for reliable quantification of exons and alternatively spliced variants. We introduce a novel, computationally efficient methodology, Multiple Exon Array Preprocessing (MEAP), for exon array data pre-processing, analysis and visualization. We compared MEAP with existing pre-processing methods, and validation of six exons and t

SUBMITTER: Chen P 

PROVIDER: S-EPMC3185423 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature