A platform for efficient genotyping in Musa using microsatellite markers.

ABSTRACT:

Background and aims

Bananas and plantains (Musa spp.) are one of the major fruit crops worldwide with acknowledged importance as a staple food for millions of people. The rich genetic diversity of this crop is, however, endangered by diseases, adverse environmental conditions and changed farming practices, and the need for its characterization and preservation is urgent. With the aim of providing a simple and robust approach for molecular characterization of Musa species, we developed an optimized genotyping platform using 19 published simple sequence repeat markers.

Methodology

The genotyping system is based on 19 microsatellite loci, which are scored using fluorescently labelled primers and high-throughput capillary electrophoresis separation with high resolution. This gen

SUBMITTER: Christelova P 

PROVIDER: S-EPMC3185971 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature