Assigning statistical significance to proteotypic peptides via database searches.

ABSTRACT: Querying MS/MS spectra against a database containing only proteotypic peptides reduces data analysis time due to reduction of database size. Despite the speed advantage, this search strategy is challenged by issues of statistical significance and coverage. The former requires separating systematically significant identifications from less confident identifications, while the latter arises when the underlying peptide is not present, due to single amino acid polymorphisms (SAPs) or post-translational modifications (PTMs), in the proteotypic peptide libraries searched. To address both issues simultaneously, we have extended RAId's knowledge database to include proteotypic information, utilized RAId's statistical strategy to assign statistical significance to proteotypic peptides, and modified

SUBMITTER: Alves G 

PROVIDER: S-EPMC3186061 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature