Association of ActA to peptidoglycan revealed by cell wall proteomics of intracellular Listeria monocytogenes.

ABSTRACT: Listeria monocytogenes is a Gram-positive intracellular bacterial pathogen that colonizes the cytosol of eukaryotic cells. Recent transcriptomic studies have revealed that intracellular L. monocytogenes alter expression of genes encoding envelope components. However, no comparative global analysis of this cell wall remodeling process is yet known at the protein level. Here, we used high resolution mass spectrometry to define the cell wall proteome of L. monocytogenes growing inside epithelial cells. When compared with extracellular bacteria growing in a nutrient-rich medium, a major difference found in the proteome was the presence of the actin assembly-inducing protein ActA in peptidoglycan purified from intracellular bacteria. ActA was also identified in the peptidoglycan of extracellula

SUBMITTER: Garcia-del Portillo F 

PROVIDER: S-EPMC3186376 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature