SigmaB- and PrfA-dependent transcription of genes previously classified as putative constituents of the Listeria monocytogenes PrfA regulon.

ABSTRACT: Mounting evidence suggests that sigma(B) and PrfA coregulate transcription of multiple genes in Listeria monocytogenes, therefore, the relative contributions of sigma(B) and PrfA to transcript levels of genes identified previously as differentially regulated by PrfA were measured. Group I genes are recognized virulence genes that are positively regulated by PrfA; group II genes were reported previously as negatively regulated by PrfA; and multiple group III genes were proposed to be coregulated by sigma(B) and PrfA. Transcript levels for selected genes were measured by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in L. monocytogenes 10403S as well as in otherwise isogenic DeltasigB, DeltaprfA, and DeltasigBDeltaprfA strains grown under conditions demonstrated to in

SUBMITTER: Ollinger J 

PROVIDER: S-EPMC3186697 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature