Matrix metalloproteinase-9 inhibition reduces inflammation and improves motility in murine models of postoperative ileus.

ABSTRACT:

Background & aims

Matrix metalloproteinase (MMP)-9, a member of the gelatinase family of MMPs, mediates leukocyte migration during inflammation. Inflammation contributes to development of postoperative ileus (POI), which is caused by physical disturbances to the bowel during abdominal surgery. We evaluated the role of MMP-9 in POI and investigated whether disruption of MMP-9 or administration of an inhibitor of MMP-9 activity reduced cellular inflammation and bowel dysmotility in rat and mouse models of POI.

Methods

Mice and rats underwent laparotomy and bowel manipulation; bowel tissues were collected 3 to 24 hours later and analyzed by real-time reverse-transcriptase polymerase chain reaction, immunoblot, in situ zymography, and functional analyses.

Results

Bowel ma

SUBMITTER: Moore BA 

PROVIDER: S-EPMC3186882 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature