Ncb2 is involved in activated transcription of CDR1 in azole-resistant clinical isolates of Candida albicans.

ABSTRACT: We recently demonstrated that CDR1 overexpression in azole-resistant isolates of Candida albicans is due to its enhanced transcriptional activation and increased mRNA stability. In this study, we provide the first evidence of transcriptional regulation of CDR1 by Ncb2, the β subunit of NC2, a heterodimeric regulator of transcription. Conditional NCB2 null mutants displayed decreased susceptibility toward azole and an enhanced transcription of CDR1. Interestingly, Ncb2 associated with the CDR1 promoter under both repression and activation; however, an increase in recruitment was observed under both transient and constitutive activation states. By chromatin immunoprecipitation (ChIP) assay, we showed the preferential recruitment of Ncb2 to the core TATA region under activation (azole-resista

SUBMITTER: Shukla S 

PROVIDER: S-EPMC3187062 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature