Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation.

ABSTRACT: A novel technically compliant expression system was developed for heterologous protein production in Bacillus subtilis with the aim of increasing product yields at the same time as decreasing production costs. Standard systems involve the positively regulated manP promoter of the mannose operon, which led to relatively high product yields of 5.3% (5.3 g enhanced green fluorescent protein [eGFP] per 100 g cell dry weight [CDW]) but required large quantities of mannose to induce the reactions, thus rendering the system's technical application rather expensive. To improve this situation, mutant B. subtilis strains were used: the ΔmanA (mannose metabolism) strain TQ281 and the ΔmanP (mannose uptake) strain TQ356. The total amount of inducer could be reduced with TQ281, which, however, displaye

SUBMITTER: Wenzel M 

PROVIDER: S-EPMC3187127 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature