Real-time detection and identification of Chlamydophila species in veterinary specimens by using SYBR green-based PCR assays.

ABSTRACT: Infections caused by members of the Chlamydiaceae family have long been underestimated due to the requirement of special laboratory facilities for the detection of this group of intracellular pathogens. Furthermore, new studies of this group of intracellular pathogens have revealed that host specificity of different species is not as clear as recently believed. As most members of the genus Chlamydophila have shown to be transmissible from animals to humans, sensitive and fast detection methods are required. In this study, SYBR green-based real-time assays were developed that detect all members of Chlamydiaceae and differentiate the most prevalent veterinary Chlamydophila species: Cp. psittaci, Cp. abortus, Cp. felis, and Cp. caviae. By adding bovine serum albumin to the master mixes, targe

SUBMITTER: Nordentoft S 

PROVIDER: S-EPMC3187146 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature