Isolation of generalized transducing bacteriophages for uropathogenic strains of Escherichia coli.

ABSTRACT: The traditional genetic procedure for random or site-specific mutagenesis in Escherichia coli K-12 involves mutagenesis, isolation of mutants, and transduction of the mutation into a clean genetic background. The transduction step reduces the likelihood of complications due to secondary mutations. Though well established, this protocol is not tenable for many pathogenic E. coli strains, such as uropathogenic strain CFT073, because it is resistant to known K-12 transducing bacteriophages, such as P1. CFT073 mutants generated via a technique such as lambda Red mutagenesis may contain unknown secondary mutations. Here we describe the isolation and characterization of transducing bacteriophages for CFT073. Seventy-seven phage isolates were acquired from effluent water samples collected from a

SUBMITTER: Battaglioli EJ 

PROVIDER: S-EPMC3187168 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature