Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.

ABSTRACT: Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

SUBMITTER: Abraham S 

PROVIDER: S-EPMC3187172 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature