Proliferation of dental follicle-derived cell populations in heat-stress conditions.

ABSTRACT:

Objectives

Isolation and purification of adult stem cells (ASC) are a great challenge. Our objectives were to determine whether ASC are more heat-tolerant than non-stem cells, and to explore if ASC could be enriched by heat-stress treatments.

Materials and methods

Rat dental follicle cells were cultured in a variety of media to obtain either a heterogeneous cell population (H-DFC) consisting of stem cells and non-stem cells, or a homogenous cell population (DFC) containing non-stem cells only. Real-time RT-PCR was conducted to compare expression of heat-shock proteins (HSPs) between the two populations. To study heat tolerance, H-DFC and DFC were incubated under heat-stress conditions and cell proliferation was evaluated by alamar blue reduction assay. Furthermore, cells res

SUBMITTER: Yao S 

PROVIDER: S-EPMC3187570 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature