Spatial segregation of BMP/Smad signaling affects osteoblast differentiation in C2C12 cells.

ABSTRACT:

Background

Bone morphogenetic proteins (BMPs) are involved in a plethora of cellular processes in embryonic development and adult tissue homeostasis. Signaling specificity is achieved by dynamic processes involving BMP receptor oligomerization and endocytosis. This allows for spatiotemporal control of Smad dependent and non-Smad pathways. In this study, we investigate the spatiotemporal regulation within the BMP-induced Smad transcriptional pathway.

Methodology/principal findings

Here we discriminate between Smad signaling events that are dynamin-dependent (i.e., require an intact endocytic pathway) and dynamin-independent. Inhibition of dynamin-dependent endocytosis in fluorescence microscopy and fractionation studies revealed a delay in Smad1/5/8 phosphorylation and nuclea

SUBMITTER: Heining E 

PROVIDER: S-EPMC3187766 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature