Gene expression of human lung cancer cell line CL1-5 in response to a direct current electric field.

ABSTRACT:

Background

Electrotaxis is the movement of adherent living cells in response to a direct current (dc) electric field (EF) of physiological strength. Highly metastatic human lung cancer cells, CL1-5, exhibit directional migration and orientation under dcEFs. To understand the transcriptional response of CL1-5 cells to a dcEF, microarray analysis was performed in this study.

Methodology/principal findings

A large electric-field chip (LEFC) was designed, fabricated, and used in this study. CL1-5 cells were treated with the EF strength of 0 mV/mm (the control group) and 300 mV/mm (the EF-treated group) for two hours. Signaling pathways involving the genes that expressed differently between the two groups were revealed. It was shown that the EF-regulated genes highly correlated t

SUBMITTER: Huang CW 

PROVIDER: S-EPMC3187831 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature