Paeoniflorin suppresses the expression of intercellular adhesion molecule-1 (ICAM-1) in endotoxin-treated human monocytic cells.

ABSTRACT:

Background and purpose

Paeoniflorin (PF) has ameliorative effects on learning and memory impairment and cerebral ischaemia in rats and has protective effects against the degeneration of dopaminergic neurons in substantia nigra. The neuroprotective effects of PF are most probably derived from its anti-inflammatory property. Abnormally high levels of intercellular adhesion molecule-1 (ICAM-1) have been found to be associated with a wide range of inflammatory and immune responses. Here we studied whether PF regulates the levels of ICAM-1 elevated in LPS-activated differentiated human monocytic U937 cells and TNF-α-stimulated human umbilical vein endothelial cells (HUVECs).

Experimental approach

mRNA levels were evaluated by RT-PCR. Protein levels were evaluated by Western blot

SUBMITTER: Jin L 

PROVIDER: S-EPMC3188904 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature