On the mode of action of emodepside: slow effects on membrane potential and voltage-activated currents in Ascaris suum.

ABSTRACT:

Background and purpose

Anthelmintics are required for treatment and prophylaxis of nematode parasites of humans and domestic animals. Emodepside, a cyclooctadepsipeptide, is a modern anthelmintic that has a novel mode of action involving a Ca-activated K channel (SLO-1) in Caenorhabditis elegans, sometimes mediated by a latrophilin (LAT) receptor. We examined mechanisms of action of emodepside in a parasitic nematode, Ascaris suum.

Experimental approach

RT-PCR was used to investigate expression of slo-1 and lat-1 in A. suum muscle flaps, and two-micropipette current-clamp and voltage-clamp techniques were used to record electrophysiological effects of emodepside.

Key results

Expression of slo-1 and lat-1 were detected. Emodepside produced a slow time-dependent (20 min

SUBMITTER: Buxton SK 

PROVIDER: S-EPMC3188918 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature