Pharmacological properties of the rhesus macaque monkey P2X7 receptor.

ABSTRACT:

Background and purpose

The human P2X7 (hP2X7) receptor exhibits striking pharmacological differences from its rodent counterparts, particularly in terms of its antagonist profile. Here, we characterized the functional and pharmacological properties of the rhesus macaque monkey P2X7 (rmP2X7) receptor in comparison with the hP2X7 receptor.

Experimental approach

The rmP2X7 and hP2X7 receptors were heterologously expressed in HEK293 cells. The receptor surface and total expression levels were examined by biotin-labelling and Western blotting. The functional and pharmacological properties were characterized using patch-clamp recording and single-cell imaging.

Key results

The rmP2X7 receptor showed strong cell surface expression. Both ATP and 2'(3')-O-(4-benzoylbenzoyl) ade

SUBMITTER: Bradley HJ 

PROVIDER: S-EPMC3188921 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature