Analysis of a Drosophila amplicon in follicle cells highlights the diversity of metazoan replication origins.

ABSTRACT: To investigate the properties of metazoan replication origins, recent studies in cell culture have adopted the strategy of identifying origins using genome-wide approaches and assessing correlations with such features as transcription and histone modifications. Drosophila amplicon in follicle cells (DAFCs), genomic regions that undergo repeated rounds of DNA replication to increase DNA copy number, serve as powerful in vivo model replicons. Because there are six DAFCs, compared with thousands of origins activated in the typical S phase, close molecular characterization of all DAFCs is possible. To determine the extent to which the six DAFCs are different or similar, we investigated the developmental and replication properties of the newly identified DAFC-34B. DAFC-34B contains two genes ex

SUBMITTER: Kim JC 

PROVIDER: S-EPMC3189023 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature