High resolution of microRNA signatures in human whole saliva.

ABSTRACT:

Objective

Identifying discriminatory human salivary RNA biomarkers reflective of disease in a low-cost non-invasive screening assay is crucial to salivary diagnostics. Recent studies have reported both mRNA and microRNA (miRNA) in saliva, but little information has been documented on the quality and yield of RNA collected. Therefore, the aim of the present study was to develop an improved RNA isolation method from saliva and to identify major miRNA species in human whole saliva.

Design

RNA samples were isolated from normal human saliva using a combined protocol based on the Oragene RNA collection kit and the mirVana miRNA isolation kit in tandem. RNA samples were analysed for quality and subjected to miRNA array analysis.

Results

RNA samples isolated from twenty healt

SUBMITTER: Patel RS 

PROVIDER: S-EPMC3189266 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature