Characterization of multiprotein complexes of the Borrelia burgdorferi outer membrane vesicles.

ABSTRACT: Among bacterial cell envelopes, the Borrelia burgdorferi outer membrane (OM) is structurally unique in that the identities of many protein complexes remain unknown; however, their characterization is the first step toward our understanding of membrane protein interactions and potential functions. Here, we used two-dimensional blue native/SDS-PAGE/mass spectrometric analysis for a global characterization of protein-protein interactions as well as to identify protein complexes in OM vesicles isolated from multiple infectious sensu stricto isolates of B. burgdorferi. Although we uncovered the existence of at least 10 distinct OM complexes harboring several unique subunits, the complexome is dominated by the frequent occurrence of a limited diversity of membrane proteins, most notably P13, out

SUBMITTER: Yang X 

PROVIDER: S-EPMC3189302 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature