Differential proteome analysis of human embryonic kidney cell line (HEK-293) following mycophenolic acid treatment.

ABSTRACT:

Background

Mycophenolic acid (MPA) is widely used as a post transplantation medicine to prevent acute organ rejection. In the present study we used proteomics approach to identify proteome alterations in human embryonic kidney cells (HEK-293) after treatment with therapeutic dose of MPA. Following 72 hours MPA treatment, total protein lysates were prepared, resolved by two dimensional gel electrophoresis and differentially expressed proteins were identified by QTOF-MS/MS analysis. Expressional regulations of selected proteins were further validated by real time PCR and Western blotting.

Results

The proliferation assay demonstrated that therapeutic MPA concentration causes a dose dependent inhibition of HEK-293 cell proliferation. A significant apoptosis was observed after MP

SUBMITTER: Qasim M 

PROVIDER: S-EPMC3189873 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature