Extracellular ATP activates NFAT-dependent gene expression in neuronal PC12 cells via P2X receptors.

ABSTRACT:

Background

Treatment of neuronal PC12 cells with ATP induces depolarisation and increases intracellular calcium levels via purinergic receptors. In many cell types, sustained elevation of intracellular calcium levels cause changes in gene expression via activation of the transcription factor NFAT (nuclear factor of activated T cells). We have therefore characterised the signalling pathway by which ATP regulates NFAT-dependent gene expression in PC12 cells.

Results

The activation of NFAT transcriptional activity by extracellular ATP was characterised with the help of reporter gene assays. Treatment of PC12 cells with ATP elicited a dose-dependent increase in luciferase activity (EC₅₀ = 78 μM). UTP, 4-benzoylbenzoyl ATP and α,β-methylene ATP did not mimic the effect of ATP, wh

SUBMITTER: Prasai P 

PROVIDER: S-EPMC3189881 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature