Flow injection measurements of S-nitrosothiols species in biological samples using amperometric nitric oxide sensor and soluble organoselenium catalyst reagent.

ABSTRACT: A novel flow injection analysis (FIA) system suitable for measurement of S-nitrosothiols (RSNOs) in blood plasma is described. In the proposed (FIA) system, samples and standards containing RSNO species are injected into a buffer carrier stream that is mixed with the reagent stream containing 3,3'-dipropionicdiselenide (SeDPA) and glutathione (GSH). SeDPA has been shown previously to catalytically decompose RSNOs in the presence of a reducing agent, such as GSH, to produce nitric oxide (NO). The liberated NO is then detected downstream by an amperometric NO sensor. This sensor is prepared using an electropolymerized m-phenylenediamine (m-PD)/resorcinol and Nafion composite films at the surface of a platinum electrode. Using optimized flow rates and reagent concentrations, detection of vari

SUBMITTER: Huang C 

PROVIDER: S-EPMC3190598 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature