BM-map: Bayesian mapping of multireads for next-generation sequencing data.

ABSTRACT: Next-generation sequencing (NGS) technology generates millions of short reads, which provide valuable information for various aspects of cellular activities and biological functions. A key step in NGS applications (e.g., RNA-Seq) is to map short reads to correct genomic locations within the source genome. While most reads are mapped to a unique location, a significant proportion of reads align to multiple genomic locations with equal or similar numbers of mismatches; these are called multireads. The ambiguity in mapping the multireads may lead to bias in downstream analyses. Currently, most practitioners discard the multireads in their analysis, resulting in a loss of valuable information, especially for the genes with similar sequences. To refine the read mapping, we develop a Bayesian mo

SUBMITTER: Ji Y 

PROVIDER: S-EPMC3190637 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature