Comprehensive analysis of pathogen-specific antibody response in vivo based on an antigen library displayed on surface of yeast.

ABSTRACT: Host antibody response is a crucial defense against pathogenic infection. Here, we report a novel technique allowing quantitative measurement of polyclonal antibody response in vivo. This involves expression of a combinatorial library of target proteins from a candidate pathogen on the surface of yeast Saccharomyces cerevisiae. After mixing with serum/plasma from infected or immunized subjects, positive yeast clones were isolated via fluorescence-activated cell sorting (FACS). Using this technique, we have studied mouse immunized serum with recombinant hemagglutinin (HA) protein from a human influenza H5N1 strain (A/Anhui/1/2005) and convalescent plasma from an infected human in China. Our technique has identified novel antigenic domains targeted by serum/plasma and allowed calculation of

SUBMITTER: Zuo T 

PROVIDER: S-EPMC3190923 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature