Genetic manipulation of Schistosoma haematobium, the neglected schistosome.

ABSTRACT:

Background

Minimal information on the genome and proteome of Schistosoma haematobium is available, in marked contrast to the situation with the other major species of human schistosomes for which draft genome sequences have been reported. Accordingly, little is known about functional genomics in S. haematobium, including the utility or not of RNA interference techniques that, if available, promise to guide development of new interventions for schistosomiasis haematobia.

Methods/findings

Here we isolated and cultured developmental stages of S. haematobium, derived from experimentally infected hamsters. Targeting different developmental stages, we investigated the utility of soaking and/or square wave electroporation in order to transfect S. haematobium with nucleic acid repor

SUBMITTER: Rinaldi G 

PROVIDER: S-EPMC3191139 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature