Downregulation of E-Cadherin enhances proliferation of head and neck cancer through transcriptional regulation of EGFR.

ABSTRACT:

Background

Epidermal growth factor receptor (EGFR) has been reported to downregulate E-cadherin (E-cad); however, whether the downregulation of E-cad has any effect on EGFR expression has not been elucidated. Our previous studies have found an inverse correlation between EGFR and E-cad expression in tissue specimens of squamous cell carcinoma of the head and neck (SCCHN). To understand the biological mechanisms underlying this clinical observation, we knocked down E-cad expression utilizing E-cad siRNA in four SCCHN cell lines.

Results

It was observed that downregulation of E-cad upregulated EGFR expression compared with control siRNA-transfected cells after 72 hours. Cellular membrane localization of EGFR was also increased. Consequently, downstream signaling molecules of t

SUBMITTER: Wang D 

PROVIDER: S-EPMC3192774 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature