Identification and validation of rice reference proteins for western blotting.

ABSTRACT: Studies of rice protein expression have increased considerably with the development of rice functional genomics. In order to obtain reliable expression results in western blotting, information on appropriate reference proteins is necessary for data normalization. To date, no published study has identified and systematically validated reference proteins suitable for the investigation of rice protein expression. In this study, nine candidate proteins were selected and their specific antibodies were obtained through immunization of rabbits with either recombinant proteins expressed in Escherichia coli or synthesized peptides. Western blotting was carried out to detect the expression of target proteins in a set of 10 rice samples representing different rice tissues/organs at different developm

SUBMITTER: Li X 

PROVIDER: S-EPMC3192993 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature