Yeast pheromone receptor genes STE2 and STE3 are differently regulated at the transcription and polyadenylation level.

ABSTRACT: The orderly expression of specific genes is the basis for cell differentiation. Saccharomyces cerevisiae has two haploid mating types, a and α cells, in which the mating-specific genes are differentially expressed. When a and α cells are committed to mate, their growth is arrested. Here we show that a cryptic polyadenylation site is present inside the coding region of the a-specific STE2 gene, encoding the receptor for the α-factor. The two cell types produce an incomplete STE2 transcript, but only a cells generate full-length STE2 mRNA. We eliminated the cryptic poly(A) signal, thereby allowing the production of a complete STE2 mRNA in α cells. We mutagenized α cells and isolated a mutant producing full-length STE2 mRNA. The mutation occurred in the ITC1 gene, whose product, together with

SUBMITTER: Di Segni G 

PROVIDER: S-EPMC3193217 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature