Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samples.

ABSTRACT: Conventional tools for measuring dietary exposure have well recognized limitations. Measurement of food-derived metabolites in biofluids provides an alternative approach and our aim was to develop an experimental protocol which ensures that extraneous variability does not obscure metabolic signals from ingested foods. Healthy adults consumed a standardized meal in the evening before each test day and collected pooled overnight urine. On each test day of three different studies, urine was collected in the fasted state and at different time points after consumption of a standardized breakfast. Metabolite fingerprinting of samples using Flow Infusion Electrospray-Ionization Mass Spectrometry followed by multivariate data analysis showed strong discrimination between overnight, fasting and pos

SUBMITTER: Fave G 

PROVIDER: S-EPMC3193537 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature