Reference genes for gene expression studies in wheat flag leaves grown under different farming conditions.

ABSTRACT:

Background

Internal control genes with highly uniform expression throughout the experimental conditions are required for accurate gene expression analysis as no universal reference genes exists. In this study, the expression stability of 24 candidate genes from Triticum aestivum cv. Cubus flag leaves grown under organic and conventional farming systems was evaluated in two locations in order to select suitable genes that can be used for normalization of real-time quantitative reverse-transcription PCR (RT-qPCR) reactions. The genes were selected among the most common used reference genes as well as genes encoding proteins involved in several metabolic pathways.

Findings

Individual genes displayed different expression rates across all samples assayed. Applying geNorm, a set o

SUBMITTER: Tenea GN 

PROVIDER: S-EPMC3193821 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature