Acid phosphatase test proves superior to standard phenotypic identification procedure for Clostridium perfringens strains isolated from water.

ABSTRACT: Clostridium perfringens is used as an indicator for persistent faecal pollution as well as to monitor the efficacy of water treatment processes. For these purposes, differentiation between C. perfringens and other Clostridia is essential and is routinely carried out by phenotypic standard tests as proposed in the ISO/CD 6461-2:2002 (ISO_LGMN: lactose fermentation, gelatine liquidation, motility and nitrate reduction). Because the ISO_LGMN procedure is time consuming and labour intensive, the acid phosphatase test was investigated as a possible and much more rapid alternative method for confirmation. The aim of our study was to evaluate and compare confirmation results obtained by these two phenotypic methods using genotypically identified strains, what to our knowledge has not been accompl

SUBMITTER: Ryzinska-Paier G 

PROVIDER: S-EPMC3195671 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature