Intermediate-conductance calcium-activated potassium channels participate in neurovascular coupling.

ABSTRACT:

Background and purpose

Controlling vascular tone involves K(+) efflux through endothelial cell small- and intermediate-conductance calcium-activated potassium channels (K(Ca)2.3 and K(Ca)3.1, respectively). We investigated the expression of these channels in astrocytes and the possibility that, by a similar mechanism, they might contribute to neurovascular coupling.

Experimental approach

Transgenic mice expressing enhanced green fluorescent protein (eGFP) in astrocytes were used to assess K(Ca)2.3 and K(Ca)3.1 expression by immunohistochemistry and RT-PCR. K(Ca) currents in eGFP-positive astrocytes were determined in situ using whole-cell patch clamp electrophysiology. The contribution of K(Ca)3.1 to neurovascular coupling was investigated in pharmacological experiments usin

SUBMITTER: Longden TA 

PROVIDER: S-EPMC3195915 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature