Design and cellular internalization of genetically engineered polypeptide nanoparticles displaying adenovirus knob domain.

ABSTRACT: Hepatocytes and acinar cells exhibit high-efficiency, fiber-dependent internalization of adenovirus; however, viral capsids have unpredictable immunological effects and are challenging to develop into targeted drug carriers. To exploit this internalization pathway and minimize the use of viral proteins, we developed a simple gene product that self assembles nanoparticles decorated with the knob domain of adenovirus serotype 5 fiber protein. The most significant advantages of this platform include: (i) compatibility with genetic engineering; (ii) no bioconjugate chemistry is required to link fusion proteins to the nanoparticle surface; and (iii) it can direct the reversible assembly of large nanoparticles, which are monodisperse, multivalent, and biodegradable. These particles are predomina

SUBMITTER: Sun G 

PROVIDER: S-EPMC3196066 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature