Molecular characterization of the tumor-suppressive function of nischarin in breast cancer.

ABSTRACT:

Background

Nischarin (encoded by NISCH), an α5 integrin-binding protein, has been identified as a regulator of breast cancer cell invasion. We hypothesized that it might be a tumor suppressor and were interested in its regulation.

Methods

We examined nischarin expression in approximately 300 human breast cancer and normal tissues using quantitative polymerase chain reaction and immunohistochemistry. Loss of heterozygosity analysis was performed by examining three microsatellite markers located near the NISCH locus in normal and tumor tissues. We generated derivatives of MDA-MB-231 human metastatic breast cancer cells that overexpressed nischarin and measured tumor growth from these cells as xenografts in mice; metastasis by these cells after tail vein injection; and α5 integ

SUBMITTER: Baranwal S 

PROVIDER: S-EPMC3196482 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature