Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.

ABSTRACT:

Background & aims

In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the removal of the nasogastric tube, (2) whether they can be grown in culture in vitro, and (3) whether the physiological state of exfoliated cells expressing H+/K+ -ATPases reflects that of their counterparts remaining in situ at the surface of the gastric epithelium in neonatal rat pups.

Methods

In infants, gastric fluid aspirates were collected weekly after birth or every 3 hours over 24-h periods, and related to clinical parameters (Biocollection PROG/09/18). In rat pups submitted to a single fasting/refeedi

SUBMITTER: Kaeffer B 

PROVIDER: S-EPMC3196493 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature