Fluorescent peptide biosensor for probing the relative abundance of cyclin-dependent kinases in living cells.

ABSTRACT: Cyclin-dependant kinases play a central role in coordinating cell growth and division, and in sustaining proliferation of cancer cells, thereby constituting attractive pharmacological targets. However, there are no direct means of assessing their relative abundance in living cells, current approaches being limited to antigenic and proteomic analysis of fixed cells. In order to probe the relative abundance of these kinases directly in living cells, we have developed a fluorescent peptide biosensor with biligand affinity for CDKs and cyclins in vitro, that retains endogenous CDK/cyclin complexes from cell extracts, and that bears an environmentally-sensitive probe, whose fluorescence increases in a sensitive fashion upon recognition of its targets. CDKSENS was introduced into living cells, t

SUBMITTER: Kurzawa L 

PROVIDER: S-EPMC3196589 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature