Identifying inhibitors of epithelial-mesenchymal transition by connectivity map-based systems approach.

ABSTRACT:

Background

Acquisition of mesenchymal phenotype by epithelial cells by means of epithelial-mesenchymal transition (EMT) is considered as an early event in the multistep process of tumor metastasis. Therefore, inhibition of EMT might be a rational strategy to prevent metastasis.

Methods

Using the global gene expression profile from a cell culture model of transforming growth factor-β (TGF-β)-induced EMT, we identified potential EMT inhibitors. We used a publicly available database (www.broad.mit.edu/cmap) comprising gene expression profiles obtained from multiple different cell lines in response to various drugs to derive negative correlations to EMT gene expression profile using Connectivity Map, a pattern matching tool.

Results

Experimental validation of the identifi

SUBMITTER: Reka AK 

PROVIDER: S-EPMC3196823 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature