Impaired adenosine-5'-triphosphate release from red blood cells promotes their adhesion to endothelial cells: a mechanism of hypoxemia after transfusion.

ABSTRACT:

Objective

Transfusion of red blood cells has been linked to disappointing clinical outcomes in the critically ill, but specific mechanisms of organ dysfunction after transfusion remain poorly understood. We tested the hypothesis that red blood cell storage impairs the ability of red blood cells to release adenosine-5'-triphosphate and that impaired adenosine-5'-triphosphate release was injurious in vivo, in part through increased red blood cell adhesion.

Design

Prospective, controlled, mechanistic study.

Setting

University research laboratory.

Subjects

Human and mouse blood donors; nude mouse transfusion recipients.

Interventions

Manipulation of adenosine-5'-triphosphate release, supplemental adenosine-5'-triphosphate, and antibodies to red blood cell an

SUBMITTER: Zhu H 

PROVIDER: S-EPMC3196852 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature