MiRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol.

ABSTRACT:

Background

It is known that some environmental chemicals affect the human endocrine system. The harmful effects of endocrine disrupting chemical (EDC) nonylphenol (NP) have been studied since the 1980s. It is known that NP adversely affects physiological functions by mimicking the natural hormone 17 beta-estradiol. In the present study, we analyzed the expression of miRNAs and their target genes in mouse Sertoli TM4 cells to better understand the regulatory roles of miRNAs on Sertoli cells after NP exposure.

Methods

Mouse TM4 Sertoli cells were treated with NP for 3 or 24 h, and global gene and miRNA expression were analyzed using Agilent mouse whole genome and mouse miRNA v13 arrays.

Results

We identified genes that were > 2-fold differentially expressed in NP-treate

SUBMITTER: Choi JS 

PROVIDER: S-EPMC3196907 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature