Sample collection and handling considerations for peptidomic studies in whole saliva; implications for biomarker discovery.

ABSTRACT:

Background

Proteomic studies in saliva have demonstrated its potential as a diagnostic biofluid, however the salivary peptidome is less studied. Here we study the effects of several sample collection and handling factors on salivary peptide abundance levels.

Methods

Salivary peptides were isolated using an ultrafiltration device and analyzed by tandem mass spectrometry. A panel of 41 peptides common after various treatments were quantified and normalized. We evaluated the effects of freezing rate of the samples, nutritional status of the donors (fed vs. fasted), and room-temperature sample degradation on peptide abundance levels. Repeatability of our sample processing method and our instrumental analysis method were investigated.

Results

Increased sample freezing rate

SUBMITTER: de Jong EP 

PROVIDER: S-EPMC3196990 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature