Community profiling and gene expression of fungal assimilatory nitrate reductases in agricultural soil.

ABSTRACT: Although fungi contribute significantly to the microbial biomass in terrestrial ecosystems, little is known about their contribution to biogeochemical nitrogen cycles. Agricultural soils usually contain comparably high amounts of inorganic nitrogen, mainly in the form of nitrate. Many studies focused on bacterial and archaeal turnover of nitrate by nitrification, denitrification and assimilation, whereas the fungal role remained largely neglected. To enable research on the fungal contribution to the biogeochemical nitrogen cycle tools for monitoring the presence and expression of fungal assimilatory nitrate reductase genes were developed. To the ~100 currently available fungal full-length gene sequences, another 109 partial sequences were added by amplification from individual culture isol

SUBMITTER: Gorfer M 

PROVIDER: S-EPMC3197165 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature