Chronic morphine alters the presynaptic protein profile: identification of novel molecular targets using proteomics and network analysis.

ABSTRACT: Opiates produce significant and persistent changes in synaptic transmission; knowledge of the proteins involved in these changes may help to understand the molecular mechanisms underlying opiate dependence. Using an integrated quantitative proteomics and systems biology approach, we explored changes in the presynaptic protein profile following a paradigm of chronic morphine administration that leads to the development of dependence. For this, we isolated presynaptic fractions from the striata of rats treated with saline or escalating doses of morphine, and analyzed the proteins in these fractions using differential isotopic labeling. We identified 30 proteins that were significantly altered by morphine and integrated them into a protein-protein interaction (PPI) network representing potent

SUBMITTER: Abul-Husn NS 

PROVIDER: S-EPMC3197197 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature