Development of an indirect competitive enzyme-linked immunosorbent assay applied to the Botrytis cinerea quantification in tissues of postharvest fruits.

ABSTRACT:

Background

Botrytis cinerea is a phytopathogenic fungus responsible for the disease known as gray mold, which causes substantial losses of fruits at postharvest. This fungus is present often as latent infection and an apparently healthy fruit can deteriorate suddenly due to the development of this infection. For this reason, rapid and sensitive methods are necessary for its detection and quantification. This article describes the development of an indirect competitive enzyme-linked immunosorbent assay (ELISA) for quantification of B. cinerea in apple (Red Delicious), table grape (pink Moscatel), and pear (William's) tissues.

Results

The method was based in the competition for the binding site of monoclonal antibodies between B. cinerea antigens present in fruit tissues and B

SUBMITTER: Fernandez-Baldo MA 

PROVIDER: S-EPMC3197504 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature