A systems biology approach reveals the role of a novel methyltransferase in response to chemical stress and lipid homeostasis.

ABSTRACT: Using small molecule probes to understand gene function is an attractive approach that allows functional characterization of genes that are dispensable in standard laboratory conditions and provides insight into the mode of action of these compounds. Using chemogenomic assays we previously identified yeast Crg1, an uncharacterized SAM-dependent methyltransferase, as a novel interactor of the protein phosphatase inhibitor cantharidin. In this study we used a combinatorial approach that exploits contemporary high-throughput techniques available in Saccharomyces cerevisiae combined with rigorous biological follow-up to characterize the interaction of Crg1 with cantharidin. Biochemical analysis of this enzyme followed by a systematic analysis of the interactome and lipidome of CRG1 mutants rev

SUBMITTER: Lissina E 

PROVIDER: S-EPMC3197675 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature