Expression of 24,426 human alternative splicing events and predicted cis regulation in 48 tissues and cell lines.

ABSTRACT: Alternative pre-messenger RNA splicing influences development, physiology and disease, but its regulation in humans is not well understood, partially because of the limited scale at which the expression of specific splicing events has been measured. We generated the first genome-scale expression compendium of human alternative splicing events using custom whole-transcript microarrays monitoring expression of 24,426 alternative splicing events in 48 diverse human samples. Over 11,700 genes and 9,500 splicing events were differentially expressed, providing a rich resource for studying splicing regulation. An unbiased, systematic screen of 21,760 4-mer to 7-mer words for cis-regulatory motifs identified 143 RNA 'words' enriched near regulated cassette exons, including six clusters of motifs r

SUBMITTER: Castle JC 

PROVIDER: S-EPMC3197713 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature